The pronunciation of Sacagawea's name is debated in the movie. Both pronunciations used are correct.
The coin trick that Larry performs for Attila the Hun is the French Drop.
Originally, Stephen Sommers was the director of the project. He eventually left due to creative differences.
While filming, Ben Stiller and Owen Wilson only had one day that they saw each other. Stiller talked to a toothpick for the Jedediah scenes, then three months later Wilson came in and filmed all of his parts.
The Vikings in the movie speak Icelandic (only one line is heard).
Visitors to the real American Museum of Natural History increased by 20% during the holiday season following this movie's opening.
The building featured in the film, which was constructed on a sound stage in Vancouver, Canada, is based on the American Museum of Natural History in New York City, external shots of which were used in the movie.