After the untimely death of an legendary storybook author named Drosselmeyer, on story remained unfinished: "The Prince and the Raven". However, he discovered that the story kept going as his spirit and the characters seeped into reality. Later onward, Drosselmeyer ran into a duck that witnesses the "Prince"'s presence moments after his enemy, The Raven escaped from the story. He changed her into a girl by the name of "Ahiru" (Literally "Duck" in Japanese) with a mysterious pendant and granted her magical powers on a few conditions, including changing back into a duck when she quacks and changing into a human again when she has contact with water. Soon, Ahiru discovers that the prince named "Myutho" was living without a heart and vowed to restore his heart as the magical being, "Princess Tutu" whenever she encounters a heart shard. However, there are multiple forces that vie to stop her, including Myutho's best friend, Fakir and Rue Kuroha, whom happens to be Myutho's girlfriend with surprises of her own...
|The main female of the story, and was changed from a duck, to a girl by Drosselmeyer. She can be able to transform into her magical persona, Princess Tutu after her self-agreement to recover the pieces of Myutho's missing heart. However, she has yet to truly confess her love to him.|
|The "Prince" of the story and was able to show very little emotion after his heart was shattered during a battle against an enemy of his. His charm and elegance in his appearance and his dancing resulted in becoming Ahiru's love interest.|
|Girlfriend of Myutho, whom wants him to only have feelings for her (or lack thereof). When she discovered that Myutho was reclaiming his feelings, she was able to transform into Tutu's rival, Princess Kraehe. Her mission is to keep Myutho from reclaiming his heart.|
|The mean-spirited best friend of Myutho. He wants Ahiru to steer claer of him after learning that she was tampering with Myutho's feelings.|
DrosselmeyerThe supposedly deceased writer of the story, whose spirit can control the story that has leaked into the real world. He vies to finish "The Prince and the Raven" within his own terms.